Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Reagents and instruments used in the study

Article Snippet: Rabbit polyclonal anti-PSD95-specific antibody , Proteintech Group , 20665-1-AP (RRID: AB_2687961).

Techniques: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Treatment with NaHS inhibits the dysregulation of neuroplasticity induced by Quin. (A, B) Immunofluorescence images showing colocalization of PSD95 (red, CoraLite594 fluorescent dye) and NeuN (green, CoraLite488 fluorescent dye) in nine coronal sections from three mice per group. PSD95 expression was significantly upregulated in neuronal cells in the Quin + NaHS group compared with the Quin group, and this effect was reversed by the addition of ML385. Scale bar: 20 μm. (C) Western blot was used to measure PSD95, SYT1, and Syn expression levels 6 days after Quin striatal injection. Quantification of PSD95, SYT1, and Syn expression levels ( n = 3 per group). The values are reported as mean ± SD. *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). NeuN: Neuronal nuclei; PSD95: postsynaptic density protein 95; Quin: quinolinic acid; Syn: synaptophysin; SYT1: synaptotagmin 1.

Article Snippet: Rabbit polyclonal anti-PSD95-specific antibody , Proteintech Group , 20665-1-AP (RRID: AB_2687961).

Techniques: Immunofluorescence, Expressing, Western Blot, Injection